3M8V

Crystal structure of the BTB domain from Kaiso/ZBTB33, form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-11-12
Detector	MAR scanner 345 mm plate
Spacegroup name	P 63 2 2
Unit cell lengths	114.797, 114.797, 62.228
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.699 - 2.700
R-factor	0.2025
Rwork	0.201
R-free	0.24420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3m4t
RMSD bond length	0.008
RMSD bond angle	1.207
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.6.3_473)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	7037
<I/σ(I)>	35.01	5.86
Completeness [%]	99.8	99.6
Redundancy	9.9	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.3	295	0.9 M ammonium citrate, 0.1 M sodium acetate pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K