3M8O
Human IgA1 Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 108 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-19 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.780, 76.300, 55.850 |
| Unit cell angles | 90.00, 105.48, 90.00 |
Refinement procedure
| Resolution | 19.289 - 1.550 |
| R-factor | 0.154 |
| Rwork | 0.153 |
| R-free | 0.18200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qny |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.249 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.039 | 0.169 |
| Number of reflections | 50274 | 3314 |
| <I/σ(I)> | 19.51 | 6.8 |
| Completeness [%] | 98.1 | 88 |
| Redundancy | 3.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 30% PEG 3000, 0.2M sodium chloride, 0.1M tris, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
