3M8M
1.05 A Structure of Manganese-free Manganese Peroxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12-10 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.08 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.570, 45.300, 52.830 |
| Unit cell angles | 90.00, 97.31, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.050 |
| R-factor | 0.116 |
| Rwork | 0.117 |
| R-free | 0.13900 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.038 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 160.000 | 1.080 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Number of reflections | 161090 | |
| <I/σ(I)> | 9 | 3.4 |
| Completeness [%] | 91.2 | 79.3 |
| Redundancy | 4.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 20% PEG 8000, 0.2 M ammonium sulfate, 0.1 M sodium cacodylate, pH 6.5, Vapor diffusion, hanging drop, temperature 298K |
