3M89
Structure of TubZ-GTP-g-S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.03 |
| Spacegroup name | H 3 |
| Unit cell lengths | 131.200, 131.200, 75.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.600 - 2.000 |
| Rwork | 0.218 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m8k |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.700 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.250 |
| Number of reflections | 29633 | |
| <I/σ(I)> | 11.5 | 3.4 |
| Completeness [%] | 90.0 | 60 |
| Redundancy | 2.7 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 50% sodium formate, plus addition of GTP-g-S, MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
