3M7K
Crystal structure of PacI-DNA Enzyme product complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0718 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 37.089, 114.927, 114.322 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.260 - 1.920 |
| R-factor | 0.173 |
| Rwork | 0.172 |
| R-free | 0.20100 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.305 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.13) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.062 | 0.266 |
| Number of reflections | 19148 | |
| <I/σ(I)> | 28.2 | |
| Completeness [%] | 98.6 | 92.4 |
| Redundancy | 13.5 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 22% PEG3K, 2.5% ethylene glycol, 10 mM MgSO4, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
