3M7G
Structure of topoisomerase domain of topoisomerase V protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 78 |
| Detector technology | CCD |
| Collection date | 2006-03-05 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 106.717, 119.390, 63.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.560 - 2.400 |
| R-factor | 0.2025 |
| Rwork | 0.200 |
| R-free | 0.24772 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Topoisomerase domain of topo-61 strcture (2CSB) |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.419 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.790 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.117 | 0.689 |
| Number of reflections | 16259 | |
| <I/σ(I)> | 8.2 | 2.2 |
| Completeness [%] | 94.2 | 95.7 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 0.1M Sodium citrate pH 5.5, 23% PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
