3M77
Crystal Structure of Plant SLAC1 homolog TehA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | H 3 |
| Unit cell lengths | 96.022, 96.022, 136.452 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.500 |
| R-factor | 0.141 |
| Rwork | 0.140 |
| R-free | 0.16600 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.587 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.079 | 0.962 |
| Number of reflections | 74619 | |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 99.3 | 87.3 |
| Redundancy | 5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 298 | 28% PEG600, 50mM Hepe-Na pH 7.8, 1mM ZnSO4, vapor diffusion, hanging drop, temperature 298K |
