3M71
Crystal Structure of Plant SLAC1 homolog TehA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | H 3 |
| Unit cell lengths | 96.012, 96.012, 136.272 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.200 |
| R-factor | 0.142 |
| Rwork | 0.141 |
| R-free | 0.16000 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.706 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.108 | 0.879 |
| Number of reflections | 145044 | |
| <I/σ(I)> | 15.9 | 1.9 |
| Completeness [%] | 99.9 | 98.2 |
| Redundancy | 7.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 298 | 28% PEG600, 50mM Hepe-Na pH7.8, 1mM ZnSO4, vapor diffusion, hanging drop, temperature 298K |
