3M70
Crystal Structure of TehB from Haemophilus influenzae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.488, 170.217, 55.321 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.950 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.23000 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.087 | 0.931 |
| Number of reflections | 33498 | |
| <I/σ(I)> | 12.5 | 4.9 |
| Completeness [%] | 96.6 | 74.8 |
| Redundancy | 13.8 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 298 | 25% PEG3350,100mM Bis-Tris pH 6.5, 200mM Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
