3M6J
Crystal structure of unknown function protein from Leptospirillum rubarum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.843, 58.275, 93.279 |
| Unit cell angles | 90.00, 100.99, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.17907 |
| Rwork | 0.177 |
| R-free | 0.21658 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.370 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.108 | 0.495 |
| Number of reflections | 43384 | |
| <I/σ(I)> | 42.9 | 6.68 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.2 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 297 | 0.1M Bis-Tris, 0.2M MgCl2, 25% PEG3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
