3M6A
Crystal structure of Bacillus subtilis Lon C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537,1.2546 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.382, 127.400, 148.993 |
| Unit cell angles | 90.00, 100.50, 90.00 |
Refinement procedure
| Resolution | 29.915 - 3.400 |
| R-factor | 0.2673 |
| Rwork | 0.265 |
| R-free | 0.31270 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.298 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: dev_249)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.420 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.089 | 0.578 |
| Number of reflections | 52431 | |
| <I/σ(I)> | 8 | 2.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 292 | 0.1M Sodium Citrate, 0.5M Ammonium Acetate, 14% PEG 4000, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
