3M66
Crystal structure of human Mitochondrial Transcription Termination Factor 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Detector technology | CCD |
| Collection date | 2009-10-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9729 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.230, 32.240, 70.220 |
| Unit cell angles | 90.00, 106.59, 90.00 |
Refinement procedure
| Resolution | 41.790 - 1.600 |
| R-factor | 0.1746 |
| Rwork | 0.173 |
| R-free | 0.19900 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.456 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 39570 | |
| <I/σ(I)> | 14.85 | 1.92 |
| Completeness [%] | 98.7 | 98.1 |
| Redundancy | 4.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.2 | 296 | 100 mM sodium/potassium phosphate [pH 6.2], 200 mM sodium chloride, 30 % (w/v) polyethylene glycol (PEG) 300, and 1 mM dithiothreitol (DTT), VAPOR DIFFUSION, temperature 296K |
