3M5U
Crystal Structure of Phosphoserine Aminotransferase from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-21 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.738, 105.865, 70.009 |
| Unit cell angles | 90.00, 99.22, 90.00 |
Refinement procedure
| Resolution | 34.857 - 2.152 |
| R-factor | 0.172 |
| Rwork | 0.169 |
| R-free | 0.22000 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.355 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 42606 | |
| <I/σ(I)> | 10.7 | 2.94 |
| Completeness [%] | 99.7 | 97.3 |
| Redundancy | 4.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M tri-Lithium Citrate 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
