3M5P
Glucose-6-phosphate isomerase from Francisella tularensis complexed with fructose-6-phosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-25 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 114.272, 114.272, 83.723 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.100 - 1.650 |
| R-factor | 0.132 |
| Rwork | 0.131 |
| R-free | 0.16000 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.453 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.100 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.088 | 0.664 |
| Number of reflections | 76000 | |
| <I/σ(I)> | 11.5 | 2.14 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 8.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 289 | 0.2 M sodium/potassium phosphate buffer, 35% MPD, 10 mM fructose-6-phosphate, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
