3M5G
Crystal structure of a H7 influenza virus hemagglutinin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-08-12 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.882, 115.434, 250.967 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.600 |
R-factor | 0.21879 |
Rwork | 0.217 |
R-free | 0.25559 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB Entry 1TI8 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.905 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 61857 | |
<I/σ(I)> | 39.6 | 2 |
Completeness [%] | 99.2 | 99 |
Redundancy | 7.2 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch under oil | 7.2 | 298 | 20% PEG 3350, 0.2 M magnesium chloride at pH 7.2, Microbatch under oil, temperature 298K |