3M52
Crystal structure of the BTB domain from the Miz-1/ZBTB17 transcription regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.023, 80.935, 95.713 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.200 - 2.590 |
| R-factor | 0.22688 |
| Rwork | 0.225 |
| R-free | 0.26650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Single chain poly-alanine model of PDB code 1R29 residues 7-128 except where residues were identical between Miz1 and 1R29 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.145 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.700 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Number of reflections | 8404 | |
| <I/σ(I)> | 10.87 | 3.55 |
| Completeness [%] | 98.5 | 48.9 |
| Redundancy | 3.67 | 0.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 75 mM zinc acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
