3M51
Structure of the 14-3-3/PMA2 complex stabilized by Pyrrolidone1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00749 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 97.440, 97.440, 214.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.740 - 3.250 |
| R-factor | 0.312 |
| Rwork | 0.310 |
| R-free | 0.34000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yz5 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.646 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.740 | 3.500 | |
| High resolution limit [Å] | 3.250 | 20.000 | 3.250 |
| Rmerge | 0.061 | 0.025 | 0.470 |
| Number of reflections | 8499 | 49 | 1636 |
| <I/σ(I)> | 22.39 | 56.8 | 4.6 |
| Completeness [%] | 99.9 | 90.7 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277.15 | 0.1M CHES, 1.0M Na-Citrat, 30%(w/v) sucrose, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
