3M51

Structure of the 14-3-3/PMA2 complex stabilized by Pyrrolidone1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00749
Spacegroup name	I 41 2 2
Unit cell lengths	97.440, 97.440, 214.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.740 - 3.250
R-factor	0.312
Rwork	0.310
R-free	0.34000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yz5
RMSD bond length	0.002
RMSD bond angle	0.646
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.740		3.500
High resolution limit [Å]	3.250	20.000	3.250
Rmerge	0.061	0.025	0.470
Number of reflections	8499	49	1636
<I/σ(I)>	22.39	56.8	4.6
Completeness [%]	99.9	90.7	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	277.15	0.1M CHES, 1.0M Na-Citrat, 30%(w/v) sucrose, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K