3M50

Structure of the 14-3-3/PMA2 complex stabilized by Epibestatin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00749
Spacegroup name	I 41 2 2
Unit cell lengths	97.620, 97.620, 217.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.510 - 2.600
R-factor	0.259
Rwork	0.255
R-free	0.32400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yz5
RMSD bond length	0.020
RMSD bond angle	2.125
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.510		2.800
High resolution limit [Å]	2.600	20.000	2.600
Rmerge	0.063	0.033	0.544
Number of reflections	16571	48	3234
<I/σ(I)>	19.96	38.8	3.8
Completeness [%]	99.8	88.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	277.15	0.1M CHES, 1.0M Na-Citrat, 30%(w/v) sucrose, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K