3M4X
Structure of a ribosomal methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 98.556, 98.556, 106.061 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.896 - 2.280 |
| R-factor | 0.1993 |
| Rwork | 0.197 |
| R-free | 0.25040 |
| Structure solution method | SAD |
| Starting model (for MR) | 2frx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.117 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.099 | 2.350 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.082 | 0.397 |
| Number of reflections | 24384 | |
| <I/σ(I)> | 14.05 | 4.18 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.94 | 6.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | 25% Peg 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
