3M4S

Crystal structure of a putative endoribonuclease L-PSP from Entamoeba histolytica, orthorhombic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-19
Detector	RIGAKU SATURN 944+
Spacegroup name	P 21 21 2
Unit cell lengths	102.600, 110.490, 52.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.000 - 2.300
R-factor	0.216
Rwork	0.213
R-free	0.27300
Structure solution method	MR, MR
RMSD bond length	0.015
RMSD bond angle	1.443
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.080	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.085	0.498
Number of reflections	26517
<I/σ(I)>	14.58	2.6
Completeness [%]	96.4	95
Redundancy	4.8	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	INDEX SCREEN CONDITION F10: 0.2 M NaCl, 25% PEG 3350, ENHIA.00579.A AT 25.8 MG/ML, VAPOR DIFFUSION, SITTING DROP, temperature 290K