3M4R
Structure of the N-terminal Class II Aldolase domain of a conserved protein from Thermoplasma acidophilum
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-28 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97970, 0.97945 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 46.136, 46.136, 203.998 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.890 - 2.000 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.23300 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.497 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.074 | 0.622 |
| Number of reflections | 18049 | |
| <I/σ(I)> | 12.3 | 3.8 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 10.6 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.1M Bis-Tris Propane pH 7.0, 3.2M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
