3M4G
H57A HFQ from Pseudomonas Aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.460, 66.587, 68.713 |
| Unit cell angles | 91.78, 115.32, 119.92 |
Refinement procedure
| Resolution | 30.000 - 2.050 |
| R-factor | 0.2026 |
| Rwork | 0.199 |
| R-free | 0.26150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u1s |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.903 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.055 | 0.490 |
| Number of reflections | 53606 | |
| <I/σ(I)> | 14.4 | 3 |
| Completeness [%] | 97.4 | 96.5 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 4 MG/ML PROTEIN, 50 MM NACL, 100 MM NH4CL, 7,5% MMEPEG 2000, 50 MM TRIS HCL, 10 MM ZNCL2, PH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
