3M4B
A Zn-mediated tetrahedral protein lattice cage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-11 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.18 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 125.363, 125.363, 164.509 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.270 - 2.500 |
| R-factor | 0.20206 |
| Rwork | 0.198 |
| R-free | 0.25558 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.301 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.614 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.106 | 0.481 |
| Total number of observations | 13465 | |
| Number of reflections | 17431 | |
| <I/σ(I)> | 16.5 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 25% PEG 1500, 0.1 M Bis-Tris, 0.2 M CaCl2 and 5 mM ZnCl2., pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
