3M3W

Crystal structure of mouse PACSIN3 BAR domain mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9798
Spacegroup name	P 1 21 1
Unit cell lengths	47.494, 52.265, 196.461
Unit cell angles	90.00, 94.81, 90.00

Refinement procedure

Resolution	15.000 - 2.600
R-factor	0.2346
Rwork	0.232
R-free	0.28058
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.297
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.630
High resolution limit [Å]	2.600	2.600
Number of reflections	28370
Completeness [%]	94.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1