3M3M
Crystal structure of glutathione S-transferase from Pseudomonas fluorescens [Pf-5]
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 101.746, 101.746, 48.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.430 - 1.750 |
| R-factor | 0.16049 |
| Rwork | 0.159 |
| R-free | 0.19626 |
| Structure solution method | SAD |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.220 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.430 | 1.790 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.105 | 0.292 |
| Number of reflections | 24949 | |
| <I/σ(I)> | 15.4 | 2.5 |
| Completeness [%] | 99.5 | 98.37 |
| Redundancy | 10 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 60%v/v Tacsimate pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298 K |
