3M3I
Hypothetical protein from Leishmania major
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.07811 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.979, 98.429, 100.758 |
| Unit cell angles | 71.87, 80.19, 89.57 |
Refinement procedure
| Resolution | 46.720 - 2.350 |
| R-factor | 0.242 |
| Rwork | 0.239 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.223 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | BALBES |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.716 |
| High resolution limit [Å] | 2.350 |
| Number of reflections | 73773 |
| Completeness [%] | 93.0 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 293 | protein buffer 25 mM HEPES pH 7.5, 500 mM NaCl; crystallization buffer 100 mM Na Acetate, 100 mM KH2PO4, 24% PEG 1500, VAPOR DIFFUSION, temperature 293K |
