3M39
The roles of glutamates and metal ions in a rationally designed nitric oxide reductase based on myoglobin: Fe(II)-I107E FeBMb (Fe(II) binding to FeB site)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Detector technology | CCD |
| Collection date | 2009-04-29 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0890 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.760, 46.550, 74.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.650 |
| Rwork | 0.213 |
| R-free | 0.25100 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.023 |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.420 |
| High resolution limit [Å] | 1.650 |
| Rmerge | 0.110 |
| Number of reflections | 16693 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
