3M33
The crystal structure of a functionally unknown protein from Deinococcus radiodurans R1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97911, 0.97962 |
| Spacegroup name | P 62 |
| Unit cell lengths | 104.579, 104.579, 125.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.232 - 2.195 |
| R-factor | 0.1825 |
| Rwork | 0.181 |
| R-free | 0.21440 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.126 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.076 | 0.747 |
| Number of reflections | 38704 | |
| <I/σ(I)> | 42.4 | 3.25 |
| Completeness [%] | 97.0 | 96 |
| Redundancy | 8.8 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M HEPES, 1.26M (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
