3M2L

Crystal structure of the M113F mutant of alpha-hemolysin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	C 1 2 1
Unit cell lengths	151.190, 134.800, 132.890
Unit cell angles	90.00, 90.62, 90.00

Refinement procedure

Resolution	20.000 - 2.100
Rwork	0.241
R-free	0.27500
Structure solution method	FOURIER SYNTHESIS
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.100
High resolution limit [Å]	2.000
Rmerge	0.060
Number of reflections	135956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1