3M24

Crystal structure of TagBFP fluorescent protein

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-17
Detector	ADSC QUANTUM 315
Wavelength(s)	1.08
Spacegroup name	P 21 21 21
Unit cell lengths	82.953, 105.943, 137.155
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.940 - 2.200
R-factor	0.19558
Rwork	0.193
R-free	0.23803
RMSD bond length	0.009
RMSD bond angle	1.293
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.950
Rmerge	0.068
Number of reflections	65147
<I/σ(I)>	12
Completeness [%]	89.1
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	2.0 M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K