3M22
Crystal structure of TagRFP fluorescent protein
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.08 |
| Spacegroup name | I 41 |
| Unit cell lengths | 130.989, 130.989, 105.966 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.800 - 2.200 |
| R-factor | 0.161 |
| Rwork | 0.160 |
| R-free | 0.17900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.681 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.950 |
| Rmerge | 0.149 |
| Number of reflections | 50406 |
| <I/σ(I)> | 3.8 |
| Completeness [%] | 85.5 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 298 | 30% PEG 4000, 0.1 M Tris-HCl, pH 8.5, 0.2 M MgCl2, 0.1 M TCEP, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
