3M20
Crystal structure of DmpI from Archaeoglobus fulgidus determined to 2.37 Angstroms resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-10-10 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 49.070, 49.070, 118.747 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.530 - 2.370 |
Rwork | 0.296 |
R-free | 0.31600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bjp |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 24.530 | 30.000 | 2.370 |
High resolution limit [Å] | 2.290 | 4.730 | 2.290 |
Rmerge | 0.064 | 0.032 | 0.475 |
Number of reflections | 8623 | ||
<I/σ(I)> | 14.2 | ||
Completeness [%] | 96.4 | 99.4 | 97.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 28 % PEG400, 200mM CaCl2, 0.1M HEPES (pH 7.5), vapor diffusion, sitting drop, temperature 277K |