3M20
Crystal structure of DmpI from Archaeoglobus fulgidus determined to 2.37 Angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 49.070, 49.070, 118.747 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.530 - 2.370 |
| Rwork | 0.296 |
| R-free | 0.31600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bjp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 24.530 | 30.000 | 2.370 |
| High resolution limit [Å] | 2.290 | 4.730 | 2.290 |
| Rmerge | 0.064 | 0.032 | 0.475 |
| Number of reflections | 8623 | ||
| <I/σ(I)> | 14.2 | ||
| Completeness [%] | 96.4 | 99.4 | 97.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 28 % PEG400, 200mM CaCl2, 0.1M HEPES (pH 7.5), vapor diffusion, sitting drop, temperature 277K |
