3M1X
Crystal structure of a putative endoribonuclease L-PSP from Entamoeba histolytica, rhomobohedral form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-25 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 78.546, 78.546, 89.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.085 - 1.200 |
| R-factor | 0.107 |
| Rwork | 0.106 |
| R-free | 0.13100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1XRG modified with CCP4 program CHAINSAW |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.206 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.090 | 1.270 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 32477 | |
| Completeness [%] | 99.1 | 94 |
| Redundancy | 6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 290 | INDEX SCREEN CONDITION D4: 100MM CITRATE, 25% PEG 3350, ENHIA.00579.A AT 25.8 MG/ML, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
