3M1R
The crystal structure of formimidoylglutamase from Bacillus subtilis subsp. subtilis str. 168
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-19 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 142.038, 118.980, 123.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.474 - 2.199 |
| R-factor | 0.1791 |
| Rwork | 0.176 |
| R-free | 0.23020 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.154 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.500 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.100 | 0.726 |
| Number of reflections | 102844 | |
| <I/σ(I)> | 30.6 | 3.1 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 7.3 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2M MgCl2, 0.1M Sodium cacodylate, 50% (v/v) PEG200, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
