3M1F
Crosslinked complex of actin with first W domain of Vibrio parahaemolyticus VopL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Detector technology | CCD |
| Collection date | 2008-10-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.606, 76.363, 86.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.510 - 2.890 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a3z |
| RMSD bond length | 0.011 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.990 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.168 | 0.461 |
| Number of reflections | 10207 | |
| <I/σ(I)> | 16.3 | 1.8 |
| Completeness [%] | 99.2 | 92.5 |
| Redundancy | 12.9 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 298 | 0.2 N Lithium Nitrate, 20% polyethylene glycol 3350, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
