3M1E

Crystal Structure of BenM_DBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Collection date	2007-11-18
Detector	MAR
Wavelength(s)	1.0080
Spacegroup name	C 2 2 21
Unit cell lengths	33.360, 84.146, 68.734
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.070 - 1.800
R-factor	0.18778
Rwork	0.185
R-free	0.23367
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.917
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.070
High resolution limit [Å]	1.800
Number of reflections	9300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH UNDER OIL	7.5	288	Equal volumes of precipitant and protein solution. Precipitant:Crystal Screen 2 # 30 - 0.1 M HEPES pH 7.5, 10% PEG 6000, 5% MPD. Protein: 20 mM Tris base (pH 8.0), 0.5 M NaCl, 10% glycerol, 150 mM imidazole, 10 mM BME, MICROBATCH UNDER OIL, temperature 288K