3M1D
Structure of BIR1 from cIAP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 180 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 59.180, 59.180, 199.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.399 - 2.000 |
| R-factor | 0.1979 |
| Rwork | 0.196 |
| R-free | 0.23210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2poi |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.933 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.079 | 0.825 |
| Number of reflections | 12496 | |
| <I/σ(I)> | 16.6 | 2.4 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 6.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 1% PEG 4000, 0.1M LiSO4, 0.1M Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
