3M1A
The Crystal Structure of a Short-chain Dehydrogenase from Streptomyces avermitilis to 2A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-17 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 136.638, 202.779, 106.339 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.550 - 2.000 |
| R-factor | 0.2 |
| Rwork | 0.197 |
| R-free | 0.24200 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.270 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.083 | 0.574 |
| Number of reflections | 195448 | |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 98.6 | 96.5 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 289 | 30% PEG 8000, 0.1M Bis Tris pH 6.5, 0.2M Sodium acetate, 2% DMSO, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
