3M19
Crystal structure of variable lymphocyte receptor VLRA.R5.1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.333, 77.053, 65.392 |
| Unit cell angles | 90.00, 112.04, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.21175 |
| Rwork | 0.209 |
| R-free | 0.25494 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m18 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.626 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.820 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.101 | 0.322 |
| Number of reflections | 48278 | |
| <I/σ(I)> | 12.9 | 2.5 |
| Redundancy | 3.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 20% (w/v) polyethylene glycol 6000, 1.0 M LiCl and 0.1 M citric acid, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
