3M0A

Crystal structure of TRAF2:cIAP2 complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-28
Detector	ADSC QUANTUM 315
Wavelength(s)	1.2822,1.2832,1.2664
Spacegroup name	I 41
Unit cell lengths	92.123, 92.123, 85.971
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.600 - 2.610
Rwork	0.227
R-free	0.26800
Structure solution method	MAD
RMSD bond length	0.019
RMSD bond angle	1.878
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.049	0.028	0.393
Number of reflections	11032
<I/σ(I)>	15.8
Completeness [%]	88.7	95.3	46.4
Redundancy	3.6	3.6	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	8% PEG 3350, 6% Tacsimate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K