3LZZ
Crystal structures of Cupin superfamily BbDUF985 from Branchiostoma belcheri tsingtauense in apo and GDP-bound forms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 |
| Unit cell lengths | 88.860, 88.860, 168.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.500 |
| R-factor | 0.20054 |
| Rwork | 0.198 |
| R-free | 0.24125 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yud |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.067 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.220 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.053 | 0.371 |
| Number of reflections | 22168 | |
| <I/σ(I)> | 15.6 | 3.1 |
| Completeness [%] | 98.6 | 98.9 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 2.0M ammonium sulfate, 0.1M sodium acetate trihydate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 289.0K |
