3LZP

Crystal Structure Analysis of the 'as-isolated' P19 protein from Campylobacter jejuni at 1.65 A at pH 9.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	53.706, 72.533, 75.185
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.800 - 1.650
R-factor	0.15926
Rwork	0.157
R-free	0.19505
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	P6222 incomplete SeMAD model at 2.8 A resolution.
RMSD bond length	0.014
RMSD bond angle	1.494
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	REFMAC
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.000	1.740
High resolution limit [Å]	1.650	1.650
Rmerge	0.041	0.598
Number of reflections	35799
<I/σ(I)>	23.2	2.8
Completeness [%]	99.6	100
Redundancy	5.9	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298	50% POLYETHYLENE GLYCOL (PEG) 250, 0.1 M CHES (2-(N-CYCLOHEXYLAMINO) ETHANE SULFONIC ACID) BUFFER PH 9.0, CRYO FROZEN WITHOUT ANY ADDITION, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K