3LZO

Crystal Structure Analysis of the copper-reconstituted P19 protein from Campylobacter jejuni at 1.65 A at pH 10.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	55.831, 72.581, 78.817
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.340 - 1.650
R-factor	0.14118
Rwork	0.139
R-free	0.18685
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	P6222 incomplete SeMAD model at 2.8 A resolution.
RMSD bond length	0.015
RMSD bond angle	1.523
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	REFMAC
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.340	1.740
High resolution limit [Å]	1.650	1.650
Rmerge	0.040	0.661
Number of reflections	39217
<I/σ(I)>	20.2	2.4
Completeness [%]	99.5	98.7
Redundancy	4.7	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10	298	25-45% POLYETHYLENE GLYCOL (PEG) 350, 0.1 M CHES (2-(N-CYCLOHEXYLAMINO) ETHANE SULFONIC ACID) BUFFER PH 10.0, CRYO FROZEN WITHOUT ANY ADDITION, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K