3LZI
RB69 DNA Polymerase (Y567A) ternary complex with dATP Opposite 7,8-dihydro-8-oxoguanine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2009-10-01 |
Detector | Q315 |
Wavelength(s) | 1.075 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.148, 117.603, 130.681 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.150 - 2.300 |
R-factor | 0.20817 |
Rwork | 0.206 |
R-free | 0.25773 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ig9 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.220 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.083 | |
Number of reflections | 51799 | |
<I/σ(I)> | 15.32 | 0.945 |
Completeness [%] | 95.3 | 78.1 |
Redundancy | 4.2 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | micro-batch vapor-diffusion | 6.5 | 293.15 | 150 mM CaCl2, 11%(w/v) PEG 350 monomethyl ether MME, and 100 mM Na Cacodylate pH 6.5 , micro-batch vapor-diffusion, temperature 293.15K |