3LYS

Crystal Structure of the N-terminal domain of the Prophage pi2 protein 01 (integrase) from Lactococcus lactis, Northeast Structural Genomics Consortium Target KR124F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-18
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	66.704, 90.272, 156.948
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 2.800
R-factor	0.26
Rwork	0.260
R-free	0.29000
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELX (followed by SOLVE/RESOLVE)
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.060	0.135
Number of reflections	34171
<I/σ(I)>	16.6	3.9
Completeness [%]	77.4	56.6
Redundancy	4	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microbatch, under oil	6.5	291	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: 0.1M Cacodylate acid (pH 6.5) and 20% PEG8k, Microbatch, under oil, temperature 291K