3LYL
Structure of 3-oxoacyl-acylcarrier protein reductase, FabG from Francisella tularensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-07-31 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.766, 86.941, 77.522 |
| Unit cell angles | 90.00, 98.32, 90.00 |
Refinement procedure
| Resolution | 37.820 - 1.950 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.065 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.126 | 0.540 |
| Number of reflections | 68455 | |
| <I/σ(I)> | 9.98 | 2.45 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 4.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10% PEG MME 5000, 5% Tacsimate, 10mM MgCl2, 100mM Bis-Tris pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
