3LYK
Structure of stringent starvation protein A homolog from Haemophilus influenzae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 116.327, 116.327, 91.339 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.23700 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.570 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.690 | 2.100 |
| Rmerge | 0.141 | 0.081 | 0.801 |
| Number of reflections | 41914 | ||
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 9.8 | 9.1 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 25% PEG 3350, 0.2M MgCl2, Bis-Tris pH 5.5, Vapor diffusion, Sitting drop, temperature 298K |
