3LYD
Crystal structure of Putative uncharacterized protein from Jonesia denitrificans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-12 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97962 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.551, 70.879, 99.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.450 |
R-factor | 0.16567 |
Rwork | 0.164 |
R-free | 0.19198 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.467 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.480 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.065 | 0.371 |
Number of reflections | 52288 | |
<I/σ(I)> | 47.1 | 3.23 |
Completeness [%] | 98.8 | 88.8 |
Redundancy | 6.3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 0.2M MgCl2, 0.1M Bis-Tris, 25% PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |