3LYB
Structure of putative endoribonuclease(KP1_3112) from Klebsiella pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 166.557, 166.557, 166.557 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.240 - 2.660 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.24800 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.447 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 7.180 | 2.650 |
| Rmerge | 0.104 | 0.053 | 0.687 |
| Number of reflections | 22307 | ||
| <I/σ(I)> | 10.7 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 21.6 | 20.9 | 21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M Na-Hepes pH 7.5, 28% PEG 400, 02.M CaCl2, Vapor diffusion, Sitting drop, temperature 298K |
