3LY4
Crystal Structure of fluorophore-labeled Class A -lactamase PenP-E166Cb in complex with penicillin G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Collection date | 2007-08-17 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.454, 74.700, 78.989 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.500 - 1.800 |
| R-factor | 0.21499 |
| Rwork | 0.212 |
| R-free | 0.25965 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4blm |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.817 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 54.400 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 34543 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
